Leukocyte Behavior in Mesenteric Microcirculation upon Experimental By Leishmania Spp. in BALB/c Mice.

Background: We aimed to determine the cellular recruitment (leukocyte rolling and adhesion) by which the Leishmania (Viannia) braziliensis , L. ( Leishmania ) amazonensis , and L. (Leishmania ) major species in the mesenteric microcirculation of BALB/c mice.
Methods: Five experimental groups were considered: group 1 ( L. braziliensis ); group 2 ( L. amazonensis ); group 3 ( L. major ); group 4 (control group with PBS); group 5 (negative control group), analyzed 3, 6, 12, and 24 h after parasite inoculation.
Results: Infections by the different Leishmania species caused an increase in the number of rolling leukocytes: L. braziliensis a peak at 6 h; L. amazonensis and L. major a peak at 3 h. The Leishmania infections induced leukocyte adhesion: L. major and L. amazonensis showed an increase after 3 and 6 h, respectively.
Conclusion: The kinetics of cellular recruitment in Leishmania infections, leading to infection susceptibility or resistance, indicates that distinct mechanisms regulate the initial response to Leishmania infection and determine its course.
Competing Interests: Conflict of interest The authors declare that there is no conflict of interest.
(Copyright © 2021 Neitzke-Abreu et al. Published by Tehran University of Medical Sciences.)