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MicroRNA-200c-3p Negatively Regulates ATP2A2 and Promotes the Progression of Papillary Thyroid Carcinoma.
- Source :
-
Biochemical genetics [Biochem Genet] 2022 Oct; Vol. 60 (5), pp. 1676-1694. Date of Electronic Publication: 2022 Jan 25. - Publication Year :
- 2022
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Abstract
- microRNA-200c-3p (miR-200c-3p) has emerged as an important tumor growth regulator. However, its function in papillary thyroid carcinoma (PTC) is poorly understood. This study was conducted to investigate the role of miR-200c-3p in the progression of human PTC. The miR-200c-3p expression in human PTC tissues and cell lines was evaluated. The target relationship between miR-200c-3p and candidate genes was predicted through bioinformatic analysis and confirmed with a luciferase reporter assay. miRNA or gene expression was altered using transfection, and cell behavior was analyzed using CCK-8, wound healing, Transwell, and colony formation assays. The tumor-promoting effects of miR-200c-3p were evaluated by xenografting tumors with K1 cells in nude mice. The expression level of miR-200c-3p in human PTC tissues and cell lines markedly increased, and this increased expression was significantly associated with a worse overall survival. When inactivated, miR-200c-3p suppressed K1 cells' malignant behaviors, including decreasing proliferation and attenuating colony formation, migration, and invasion. Its inactivation also attenuated the development of xenografted K1 cells in nude mice. The effects of miR-200c-3p mimics on promoting the malignant behaviors of PTC cells were remarkably reversed by the overexpression of ATP2A2, as a downstream target of miR-200c-3p. miR-200c-3p acts as an oncogenic gene and promotes the malignant biological behaviors of human PTC cells, thereby directly targeting ATP2A2. This regulated axis may be used as a potential therapy of PTC.<br /> (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Animals
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation genetics
Gene Expression Regulation, Neoplastic
Humans
Mice
Mice, Nude
MicroRNAs genetics
Sarcoplasmic Reticulum Calcium-Transporting ATPases genetics
Sarcoplasmic Reticulum Calcium-Transporting ATPases metabolism
Thyroid Cancer, Papillary genetics
Thyroid Cancer, Papillary pathology
Thyroid Neoplasms genetics
Thyroid Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4927
- Volume :
- 60
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biochemical genetics
- Publication Type :
- Academic Journal
- Accession number :
- 35079913
- Full Text :
- https://doi.org/10.1007/s10528-022-10184-w