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CD1d hi PD-L1 hi CD27 + Regulatory Natural Killer Subset Suppresses Atopic Dermatitis.
- Source :
-
Frontiers in immunology [Front Immunol] 2022 Jan 05; Vol. 12, pp. 752888. Date of Electronic Publication: 2022 Jan 05 (Print Publication: 2021). - Publication Year :
- 2022
-
Abstract
- Effector and regulatory functions of various leukocytes in allergic diseases have been well reported. Although the role of conventional natural killer (NK) cells has been established, information on its regulatory phenotype and function are very limited. Therefore, the objective of this study was to investigate the phenotype and inhibitory functions of transforming growth factor (TGF)-β-producing regulatory NK (NKreg) subset in mice with MC903-induced atopic dermatitis (AD). Interestingly, the population of TGF-β-producing NK cells in peripheral blood monocytes (PBMCs) was decreased in AD patients than in healthy subjects. The number of TGF-β <superscript>+</superscript> NK subsets was decreased in the spleen or cervical lymph node (cLN), but increased in ear tissues of mice with AD induced by MC903 than those of normal mice. We further observed that TGF-β <superscript>+</superscript> NK subsets were largely included in CD1d <superscript>hi</superscript> PD-L1 <superscript>hi</superscript> CD27 <superscript>+</superscript> NK cell subset. We also found that numbers of ILC2s and T <subscript>H</subscript> 2 cells were significantly decreased by adoptive transfer of CD1d <superscript>hi</superscript> PD-L1 <superscript>hi</superscript> CD27 <superscript>+</superscript> NK subsets. Notably, the ratio of splenic Treg per T <subscript>H</subscript> 2 was increased by the adoptive transfer of CD1d <superscript>hi</superscript> PD-L1 <superscript>hi</superscript> CD27 <superscript>+</superscript> NK cells in mice. Taken together, our findings demonstrate that the TGF-β-producing CD1d <superscript>hi</superscript> PD-L1 <superscript>hi</superscript> CD27 <superscript>+</superscript> NK subset has a previously unrecognized role in suppressing T <subscript>H</subscript> 2 immunity and ILC2 activation in AD mice, suggesting that the function of TGF-β-producing NK subset is closely associated with the severity of AD in humans.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2022 Min, Koo, Noh, Lee, Jo, Lee, Kang, Hyun, Choi and Kim.)
- Subjects :
- Animals
Antigens, CD1d immunology
B7-H1 Antigen immunology
Calcitriol adverse effects
Calcitriol analogs & derivatives
Calcitriol pharmacology
Dermatitis, Atopic chemically induced
Female
Humans
Mice
T-Lymphocytes, Regulatory immunology
Th2 Cells immunology
Transforming Growth Factor beta immunology
Tumor Necrosis Factor Receptor Superfamily, Member 7 immunology
Dermatitis, Atopic immunology
Killer Cells, Natural immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 35069528
- Full Text :
- https://doi.org/10.3389/fimmu.2021.752888