A flow cytometry based approach to identify distinct coelomocyte subsets of the purple sea urchin, Strongylocentrotus purpuratus.

The sea urchin, Strongylocentrotus purpuratus, possesses at least seven distinguishable cell populations in the coelomic fluid, which vary in morphology, size, and function. Of these, the large phagocytes, small phagocytes, and red spherule cells are thought to be key to the echinoid immune response. Because there are currently no effective and rapid means of evaluating sea urchin coelomocytes, we developed a flow cytometry based approach to identify these subsets from unseparated, unstained, live cells. In particular our gating strategy distinguishes between the large phagocytes, small phagocytes, red spherule cells, and a mixed population of vibratile cells and colorless spherule cells. This flow cytometry based analysis increases the speed and improves the reliability of coelomocyte analysis compared to differential cell counts by microscopy.
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