Cryo-EM in molecular and cellular biology.

Authors :: Saibil HR
Source :: Molecular cell [Mol Cell] 2022 Jan 20; Vol. 82 (2), pp. 274-284.
Publication Year :: 2022
Abstract: This review summarizes the current state of methods and results achievable by cryo-electron microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to understand what is required and how it might help to address their research questions. It covers some of the main issues in sample preparation, microscopes and data collection, image processing, three-dimensional (3D) reconstruction, and validation and interpretation of the resulting EM density maps and atomic models.<br />Competing Interests: Declaration of interests The author declares no competing interests.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)