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Negeviruses isolated from mosquitoes in the Brazilian Amazon.

Authors :: da Silva Ribeiro AC
Martins LC
da Silva SP
de Almeida Medeiros DB
Miranda KKP
Nunes Neto JP
de Oliveira Monteiro HA
do Nascimento BLS
Junior JWR
Cruz ACR
da Costa Vasconcelos PF
Carvalho VL
Rodrigues SG
Source :: Virology journal [Virol J] 2022 Jan 21; Vol. 19 (1), pp. 17. Date of Electronic Publication: 2022 Jan 21.
Publication Year :: 2022
Abstract: Background: There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells.<br />Methods: Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed.<br />Results: Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus.<br />Conclusions: We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.<br /> (© 2022. The Author(s).)