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Freeze-Dried Curdlan/Whey Protein Isolate-Based Biomaterial as Promising Scaffold for Matrix-Associated Autologous Chondrocyte Transplantation-A Pilot In-Vitro Study.
- Source :
-
Cells [Cells] 2022 Jan 14; Vol. 11 (2). Date of Electronic Publication: 2022 Jan 14. - Publication Year :
- 2022
-
Abstract
- The purpose of this pilot study was to establish whether a novel freeze-dried curdlan/whey protein isolate-based biomaterial may be taken into consideration as a potential scaffold for matrix-associated autologous chondrocyte transplantation. For this reason, this biomaterial was initially characterized by the visualization of its micro- and macrostructures as well as evaluation of its mechanical stability, and its ability to undergo enzymatic degradation in vitro. Subsequently, the cytocompatibility of the biomaterial towards human chondrocytes (isolated from an orthopaedic patient) was assessed. It was demonstrated that the novel freeze-dried curdlan/whey protein isolate-based biomaterial possessed a porous structure and a Young's modulus close to those of the superficial and middle zones of cartilage. It also exhibited controllable degradability in collagenase II solution over nine weeks. Most importantly, this biomaterial supported the viability and proliferation of human chondrocytes, which maintained their characteristic phenotype. Moreover, quantitative reverse transcription PCR analysis and confocal microscope observations revealed that the biomaterial may protect chondrocytes from dedifferentiation towards fibroblast-like cells during 12-day culture. Thus, in conclusion, this pilot study demonstrated that novel freeze-dried curdlan/whey protein isolate-based biomaterial may be considered as a potential scaffold for matrix-associated autologous chondrocyte transplantation.
- Subjects :
- Biomarkers metabolism
Cartilage, Articular metabolism
Cell Proliferation drug effects
Cell Proliferation genetics
Cell Survival drug effects
Cell Survival genetics
Cells, Cultured
Chondrocytes cytology
Chondrocytes drug effects
Elastic Modulus
Gene Expression Regulation drug effects
Humans
Pilot Projects
Transplantation, Autologous
Biocompatible Materials pharmacology
Chondrocytes transplantation
Extracellular Matrix chemistry
Freeze Drying
Tissue Scaffolds chemistry
Whey Proteins isolation & purification
Whey Proteins pharmacology
beta-Glucans pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4409
- Volume :
- 11
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cells
- Publication Type :
- Academic Journal
- Accession number :
- 35053397
- Full Text :
- https://doi.org/10.3390/cells11020282