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The antigenicity of SARS-CoV-2 Delta variants aggregated 10 high-frequency mutations in RBD has not changed sufficiently to replace the current vaccine strain.
- Source :
-
Signal transduction and targeted therapy [Signal Transduct Target Ther] 2022 Jan 19; Vol. 7 (1), pp. 18. Date of Electronic Publication: 2022 Jan 19. - Publication Year :
- 2022
-
Abstract
- Emerging SARS-CoV-2 variants are the most serious problem for COVID-19 prophylaxis and treatment. To determine whether the SARS-CoV-2 vaccine strain should be updated following variant emergence like seasonal flu vaccine, the changed degree on antigenicity of SARS-CoV-2 variants and H3N2 flu vaccine strains was compared. The neutralization activities of Alpha, Beta and Gamma variants' spike protein-immunized sera were analysed against the eight current epidemic variants and 20 possible variants combining the top 10 prevalent RBD mutations based on the Delta variant, which were constructed using pseudotyped viruses. Meanwhile, the neutralization activities of convalescent sera and current inactivated and recombinant protein vaccine-elicited sera were also examined against all possible Delta variants. Eight HA protein-expressing DNAs elicited-animal sera were also tested against eight pseudotyped viruses of H3N2 flu vaccine strains from 2011-2019. Our results indicate that the antigenicity changes of possible Delta variants were mostly within four folds, whereas the antigenicity changes among different H3N2 vaccine strains were approximately 10-100-fold. Structural analysis of the antigenic characterization of the SARS-CoV-2 and H3N2 mutations supports the neutralization results. This study indicates that the antigenicity changes of the current SARS-CoV-2 may not be sufficient to require replacement of the current vaccine strain.<br /> (© 2022. The Author(s).)
- Subjects :
- Amino Acid Substitution
Antibodies, Neutralizing chemistry
Antibodies, Neutralizing genetics
Antibodies, Viral chemistry
Antibodies, Viral genetics
Binding Sites
COVID-19 immunology
COVID-19 virology
COVID-19 Vaccines administration & dosage
COVID-19 Vaccines chemistry
Epitopes chemistry
Epitopes genetics
Epitopes immunology
Gene Expression
Humans
Immune Sera chemistry
Influenza A Virus, H3N2 Subtype chemistry
Influenza A Virus, H3N2 Subtype genetics
Influenza A Virus, H3N2 Subtype immunology
Influenza Vaccines administration & dosage
Influenza Vaccines chemistry
Influenza Vaccines metabolism
Influenza, Human immunology
Influenza, Human prevention & control
Influenza, Human virology
Models, Molecular
Mutation
Neutralization Tests
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
SARS-CoV-2 chemistry
SARS-CoV-2 pathogenicity
Spike Glycoprotein, Coronavirus chemistry
Spike Glycoprotein, Coronavirus genetics
Viral Pseudotyping
Antibodies, Neutralizing metabolism
Antibodies, Viral metabolism
COVID-19 prevention & control
COVID-19 Vaccines metabolism
Immunogenicity, Vaccine
SARS-CoV-2 immunology
Spike Glycoprotein, Coronavirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2059-3635
- Volume :
- 7
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Signal transduction and targeted therapy
- Publication Type :
- Academic Journal
- Accession number :
- 35046385
- Full Text :
- https://doi.org/10.1038/s41392-022-00874-7