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Opsonin-Deficient Nucleoproteic Corona Endows UnPEGylated Liposomes with Stealth Properties In Vivo .

Authors :
Giulimondi F
Vulpis E
Digiacomo L
Giuli MV
Mancusi A
Capriotti AL
Laganà A
Cerrato A
Zenezini Chiozzi R
Nicoletti C
Amenitsch H
Cardarelli F
Masuelli L
Bei R
Screpanti I
Pozzi D
Zingoni A
Checquolo S
Caracciolo G
Source :
ACS nano [ACS Nano] 2022 Feb 22; Vol. 16 (2), pp. 2088-2100. Date of Electronic Publication: 2022 Jan 18.
Publication Year :
2022

Abstract

For several decades, surface grafted polyethylene glycol (PEG) has been a go-to strategy for preserving the synthetic identity of liposomes in physiological milieu and preventing clearance by immune cells. However, the limited clinical translation of PEGylated liposomes is mainly due to the protein corona formation and the subsequent modification of liposomes' synthetic identity, which affects their interactions with immune cells and blood residency. Here we exploit the electric charge of DNA to generate unPEGylated liposome/DNA complexes that, upon exposure to human plasma, gets covered with an opsonin-deficient protein corona. The final product of the synthetic process is a biomimetic nanoparticle type covered by a proteonucleotidic corona, or "proteoDNAsome", which maintains its synthetic identity in vivo and is able to slip past the immune system more efficiently than PEGylated liposomes. Accumulation of proteoDNAsomes in the spleen and the liver was lower than that of PEGylated systems. Our work highlights the importance of generating stable biomolecular coronas in the development of stealth unPEGylated particles, thus providing a connection between the biological behavior of particles in vivo and their synthetic identity.

Details

Language :
English
ISSN :
1936-086X
Volume :
16
Issue :
2
Database :
MEDLINE
Journal :
ACS nano
Publication Type :
Academic Journal
Accession number :
35040637
Full Text :
https://doi.org/10.1021/acsnano.1c07687