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Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin.
- Source :
-
NPJ science of food [NPJ Sci Food] 2022 Jan 14; Vol. 6 (1), pp. 4. Date of Electronic Publication: 2022 Jan 14. - Publication Year :
- 2022
-
Abstract
- The identification of molecular targets of bioactive food components is important to understand the mechanistic aspect of their physiological functions. Here, we have developed a screening system that enables us to determine the activation of G protein-coupled receptors (GPCRs) by food components and have identified GPR55 as a target for curcumin. Curcumin activated GPR55 and induced serum-response element- and serum-response factor-mediated transcription, which were inhibited by Rho kinase and GPR55 antagonists. Both the methoxy group and the heptadienone moiety of curcumin were required for GPR55 activation. The F190 <superscript>5.47</superscript> residue of GPR55 was important for the interaction with curcumin. The curcumin-induced secretion of glucagon-like peptide-1 in GLUTag cells was inhibited by a GPR55 antagonist. These results indicate that expression screening is a useful system to identify GPCRs as targets of food components and strongly suggest that curcumin activates GPR55 as an agonist, which is involved in the physiological function of curcumin.<br /> (© 2022. The Author(s).)
Details
- Language :
- English
- ISSN :
- 2396-8370
- Volume :
- 6
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- NPJ science of food
- Publication Type :
- Academic Journal
- Accession number :
- 35031622
- Full Text :
- https://doi.org/10.1038/s41538-021-00119-x