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in Vitro Effect of Methamphetamine on Proliferation, Differentiation and Apoptosis of Adipose Tissue Stem Cells.
- Source :
-
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques [J Pharm Pharm Sci] 2022; Vol. 25, pp. 69-76. - Publication Year :
- 2022
-
Abstract
- Purpose: Among abused substances, methamphetamine is a psychostimulant drug widely used recreationally with public health importance. This study investigated the effect of methamphetamine on proliferation, differentiation, and apoptosis of human adipose tissue stem cells (AdSCs).<br />Methods: AdSCs were isolated from human abdominal adipose tissue and were characterized for mesenchymal properties and growth kinetics. MTT assay was undertaken to assess methamphetamine toxicity on proliferation and differentiation properties and apoptosis of hAdSCs.<br />Results: Isolated cells were shown to have mesenchymal properties and a population doubling time (PDT) of 40.1 h. Following methamphetamine treatment, expressions of KI-67 and TPX2 as proliferation genes and Col1A1 and PPARg as differentiation genes decreased. Methamphetamine administration increased the expression of Bax and decreased Bcl-2 genes responsible for apoptosis.<br />Conclusions: Our data suggested when AdSCs were exposed to methamphetamine, it decreased proliferation and differentiation properties of stem cells together with an increase in apoptosis. These findings can be added to the literature, especially when methamphetamine is used recreationally for weight loss purposes.
- Subjects :
- Adipose Tissue cytology
Cell Cycle Proteins drug effects
Genes, bcl-2 drug effects
Humans
Ki-67 Antigen drug effects
Microtubule-Associated Proteins drug effects
PPAR gamma drug effects
bcl-2-Associated X Protein blood
bcl-2-Associated X Protein drug effects
Adipose Tissue drug effects
Apoptosis drug effects
Cell Differentiation drug effects
Cell Proliferation drug effects
Mesenchymal Stem Cells drug effects
Methamphetamine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1482-1826
- Volume :
- 25
- Database :
- MEDLINE
- Journal :
- Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques
- Publication Type :
- Academic Journal
- Accession number :
- 35030074
- Full Text :
- https://doi.org/10.18433/jpps31843