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Effect of Collagen Nerve Wrapping in a Rabbit Peripheral Neuropathy Model.

Authors :
Huddleston HP
Shah NV
Kurtzman JS
Dar QA
Wang H Jr
Carter J
Hayes WT
Avoricani A
Dua K
Koehler SM
Source :
Plastic and reconstructive surgery. Global open [Plast Reconstr Surg Glob Open] 2021 Nov 11; Vol. 9 (11), pp. e3919. Date of Electronic Publication: 2021 Nov 11 (Print Publication: 2021).
Publication Year :
2021

Abstract

Background: Collagen nerve wraps (CNWs) theoretically allow for improved nerve gliding and decreased perineural scarring, and create a secluded environment to allow for nerve myelination and axonal healing. The goal of this study was to investigate the effect of CNWs on nerve gliding as assessed by pull-out strength and nerve changes in a rabbit model of peripheral neuropathy.<br />Methods: Ten New Zealand rabbits were included. Sham surgery (control) was performed on left hindlimbs. To simulate compressive neuropathy, right sciatic nerves were freed of the mesoneurium, and the epineurium was sutured to the wound bed. Five rabbits were euthanized at 6 weeks [scarred nerve (SN); n = 5]. Neurolysis with CNW was performed in the remaining rabbits at 6 weeks (CNW; n = 5), which were euthanized at 22 weeks. Outcomes included peak pull-out force and histopathological markers of nerve recovery (axonal and Schwann cell counts).<br />Results: The CNW group demonstrated significantly higher pull-out forces compared with the CNW sham control group (median: 4.40N versus 0.37N, P = 0.043) and a trend toward greater peak pull-out forces compared with the SN group (median: 4.40N versus 2.01N, P = 0.076). The CNW group had a significantly higher median Schwann cell density compared with the CNW control group (CNW: 1.30 × 10 <superscript>-3</superscript> cells/μm <superscript>2</superscript> versus CNW control: 7.781 × 10 <superscript>-4</superscript> cells/μm <superscript>2</superscript> , P = 0.0431) and SN group (CNW: 1.30 × 10 <superscript>-3</superscript> cells/μm <superscript>2</superscript> versus SN: 7.31 × 10 <superscript>-4</superscript> cells/μm <superscript>2</superscript> , P = 0.009). No significant difference in axonal density was observed between groups.<br />Conclusion: Our findings suggest using a CNW does not improve nerve gliding, but may instead play a role in recruiting and/or supporting Schwann cells and their proliferation.<br />Competing Interests: Disclosure: Dr. Koehler is a committee member of the American Society for Surgery of the Hand (ASSH), a paid consultant and speaker for Integra LifeSciences, Inc, a paid consultant for Tissium, Inc., a stockholder and member of the medical advisory board for Reactiv, Inc., and a speaker for TriMed, Inc. All the other authors have no financial interests to declare in relation to the content of this article. This study did not receive any funding.<br /> (Copyright © 2021 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons.)

Details

Language :
English
ISSN :
2169-7574
Volume :
9
Issue :
11
Database :
MEDLINE
Journal :
Plastic and reconstructive surgery. Global open
Publication Type :
Academic Journal
Accession number :
35028256
Full Text :
https://doi.org/10.1097/GOX.0000000000003919