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Protective effect of chrysin on indomethacin induced gastric ulcer in rats: role of multi-pathway regulation.
- Source :
-
Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2022 Oct; Vol. 97 (7), pp. 490-503. Date of Electronic Publication: 2022 Jan 14. - Publication Year :
- 2022
-
Abstract
- We investigated the potential gastroprotective effects of chrysin on indomethacin induced gastric ulcers in rats. We used six groups of animals: control; indomethacin (Indo); reference (Ulcuran®); indomethacin + 25 mg/kg chrysin (Indo + CHR25); indomethacin + 50 mg/kg chrysin (Indo + CHR50); indomethacin + 100 mg/kg chrysin (Indo + CHR100). All doses of chrysin were given orally to rats before indomethacin. Gastric lesions were examined macroscopically and microscopically. The effects of treatment with chrysin were assessed versus a single dose of 30 mg/kg Ulcuran® (generic ranitidine) as reference standard. We also investigated gastric mucosal superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), malonaldehyde (MDA) and arginase activities, and COX-2, PGE <subscript>2</subscript> , iNOS, TNF-α, IL-1β, NFκB, MPO, Bax, caspase-3 and 8-OHdG levels. We assessed caspase-3 and Bax levels using immunohistochemistry. Compared to the control and reference groups, SOD, CAT, GPx and arginase activities and GSH levels decreased, and MDA levels increased in the indomethacin induced gastric ulcer group. iNOS, TNF-α, IL-1β, NFκB, MAPK-14, MPO, Bax and 8-OHdG levels were increased in the indomethacin treated gastric group, while COX-2 activity and PGE <subscript>2</subscript> levels were decreased. The three doses of chrysin co-administered with indomethacin increased COX-2 activity and PGE <subscript>2</subscript> levels in rats with ulcers. Chrysin exhibited gastroprotective effects on indomethacin induced gastric ulcer due to its antioxidant, anti-inflammatory and anti-apoptotic activities.
- Subjects :
- Animals
Antioxidants pharmacology
Arginase
Caspase 3
Cyclooxygenase 2 metabolism
Glutathione
Glutathione Peroxidase
Indomethacin toxicity
Prostaglandins E metabolism
Rats
Superoxide Dismutase
Tumor Necrosis Factor-alpha
bcl-2-Associated X Protein
Flavonoids pharmacology
Stomach Ulcer chemically induced
Stomach Ulcer drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1473-7760
- Volume :
- 97
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biotechnic & histochemistry : official publication of the Biological Stain Commission
- Publication Type :
- Academic Journal
- Accession number :
- 35026960
- Full Text :
- https://doi.org/10.1080/10520295.2021.2014569