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Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2022 Jan 25; Vol. 50 (2), pp. 674-683. - Publication Year :
- 2022
-
Abstract
- The design of efficient and safe gene delivery vehicles remains a major challenge for the application of gene therapy. Of the many reported gene delivery systems, metal complexes with high affinity for nucleic acids are emerging as an attractive option. We have discovered that certain metallohelices-optically pure, self-assembling triple-stranded arrays of fully encapsulated Fe-act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. They induce formation of globular DNA particles which protect the DNA from degradation by various restriction endonucleases, are of suitable size and electrostatic potential for efficient membrane transport and are successfully processed by cells. The activity is highly structure-dependent-compact and shorter metallohelix enantiomers are far less efficient than less compact and longer enantiomers.<br /> (© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Cell Line
Cell Survival
DNA ultrastructure
Ferrous Compounds chemistry
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression
Genes, Reporter
Humans
Metal Nanoparticles ultrastructure
Microscopy, Atomic Force methods
Molecular Structure
Transfection
Cations chemistry
DNA chemistry
Gene Transfer Techniques
Genetic Vectors chemistry
Genetic Vectors ultrastructure
Metal Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 35018455
- Full Text :
- https://doi.org/10.1093/nar/gkab1277