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A small RNA that cooperatively senses two stacked metabolites in one pocket for gene control.
- Source :
-
Nature communications [Nat Commun] 2022 Jan 11; Vol. 13 (1), pp. 199. Date of Electronic Publication: 2022 Jan 11. - Publication Year :
- 2022
-
Abstract
- Riboswitches are structured non-coding RNAs often located upstream of essential genes in bacterial messenger RNAs. Such RNAs regulate expression of downstream genes by recognizing a specific cellular effector. Although nearly 50 riboswitch classes are known, only a handful recognize multiple effectors. Here, we report the 2.60-Å resolution co-crystal structure of a class I type I preQ <subscript>1</subscript> -sensing riboswitch that reveals two effectors stacked atop one another in a single binding pocket. These effectors bind with positive cooperativity in vitro and both molecules are necessary for gene regulation in bacterial cells. Stacked effector recognition appears to be a hallmark of the largest subgroup of preQ <subscript>1</subscript> riboswitches, including those from pathogens such as Neisseria gonorrhoeae. We postulate that binding to stacked effectors arose in the RNA World to closely position two substrates for RNA-mediated catalysis. These findings expand known effector recognition capabilities of riboswitches and have implications for antimicrobial development.<br /> (© 2022. The Author(s).)
- Subjects :
- Base Pairing
Cloning, Molecular
Crystallography, X-Ray
Escherichia coli genetics
Escherichia coli metabolism
Gene Expression
Gene Expression Regulation, Bacterial
Genetic Vectors chemistry
Genetic Vectors metabolism
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Neisseria gonorrhoeae metabolism
Nucleic Acid Conformation
Nucleoside Q biosynthesis
Pyrimidinones metabolism
Pyrroles metabolism
RNA, Bacterial genetics
RNA, Bacterial metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Neisseria gonorrhoeae genetics
Nucleoside Q chemistry
Pyrimidinones chemistry
Pyrroles chemistry
RNA, Bacterial chemistry
RNA, Messenger chemistry
Riboswitch
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 35017488
- Full Text :
- https://doi.org/10.1038/s41467-021-27790-8