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Development of an In Vivo Probe to Track SARS-CoV-2 Infection in Rhesus Macaques.
Development of an In Vivo Probe to Track SARS-CoV-2 Infection in Rhesus Macaques.
- Source :
-
Frontiers in immunology [Front Immunol] 2021 Dec 24; Vol. 12, pp. 810047. Date of Electronic Publication: 2021 Dec 24 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Infection with the novel coronavirus, SARS-CoV-2, results in pneumonia and other respiratory symptoms as well as pathologies at diverse anatomical sites. An outstanding question is whether these diverse pathologies are due to replication of the virus in these anatomical compartments and how and when the virus reaches those sites. To answer these outstanding questions and study the spatiotemporal dynamics of SARS-CoV-2 infection a method for tracking viral spread in vivo is needed. We developed a novel, fluorescently labeled, antibody-based in vivo probe system using the anti-spike monoclonal antibody CR3022 and demonstrated that it could successfully identify sites of SARS-CoV-2 infection in a rhesus macaque model of COVID-19. Our results showed that the fluorescent signal from our antibody-based probe could differentiate whole lungs of macaques infected for 9 days from those infected for 2 or 3 days. Additionally, the probe signal corroborated the frequency and density of infected cells in individual tissue blocks from infected macaques. These results provide proof of concept for the use of in vivo antibody-based probes to study SARS-CoV-2 infection dynamics in rhesus macaques.<br />Competing Interests: Authors AD and ML are employed by Bioqual, Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2021 Madden, Arif, Becker, McRaven, Carias, Lorenzo-Redondo, Xiao, Midkiff, Blair, Potter, Martin-Sancho, Dodson, Martinelli, Todd, Villinger, Chanda, Aye, Roy, Roederer, Lewis, Veazey and Hope.)
- Subjects :
- Animals
COVID-19 pathology
Cell Line
Disease Models, Animal
Humans
Lung pathology
Lung virology
Macaca mulatta
Proof of Concept Study
Spike Glycoprotein, Coronavirus immunology
Viral Load methods
Antibodies, Monoclonal immunology
Antibodies, Neutralizing immunology
Antibodies, Viral immunology
Fluorescent Antibody Technique methods
SARS-CoV-2 growth & development
Virus Replication physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 35003140
- Full Text :
- https://doi.org/10.3389/fimmu.2021.810047