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C-di-GMP and biofilm are regulated in Pseudomonas putida by the CfcA/CfcR two-component system in response to salts.
- Source :
-
Environmental microbiology [Environ Microbiol] 2022 Jan; Vol. 24 (1), pp. 158-178. Date of Electronic Publication: 2022 Jan 19. - Publication Year :
- 2022
-
Abstract
- In Pseudomonas putida KT2440, cfcR encodes an orphan multidomain response regulator with diguanylate cyclase activity, which is responsible for the synthesis of c-di-GMP, a second messenger key in the transition from planktonic to sessile bacterial lifestyles. When overexpressed, cfcR enhances biofilm formation and causes other phenotype alterations. The cfcA gene, encoding a membrane-anchored multisensory CHASE3/GAF hybrid histidine kinase (HK), is required to develop this pleiotropic phenotype. Here we show autophosphorylation of CfcA through HisKA/HATPase&#95;c domains and then transfer of the phosphoryl group to an internal receiver (REC) domain. CfcA REC domains are nonessential for phosphotransfer from CfcA~P to the REC domain of CfcR. CfcA~P also phosphorylates the REC domain of CfcD, a second HK encoded in the same gene cluster as CfcA, which negatively regulates the CfcA/CfcR pathway. To evaluate the impact of CfcA domains on CfcR activity, a battery of mutants with in-frame domain deletions was generated, whose CfcA protein locations were also examined. CfcA membrane anchorage contributes to protein stability and CfcR activation. Salt enhances c-di-GMP levels through CfcR, a response which is hampered by alteration of a presumed ligand-binding motif in the CHASE3 sensor domain. Thus, in P. putida, c-di-GMP is salt-regulated through the CfcA/CfcR/CfcD system.<br /> (© 2022 Society for Applied Microbiology and John Wiley & Sons Ltd.)
- Subjects :
- Bacterial Proteins genetics
Bacterial Proteins metabolism
Biofilms
Cyclic GMP analogs & derivatives
Cyclic GMP metabolism
Gene Expression Regulation, Bacterial
Phosphorus-Oxygen Lyases genetics
Phosphorus-Oxygen Lyases metabolism
Salts
Escherichia coli Proteins genetics
Pseudomonas putida genetics
Pseudomonas putida metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1462-2920
- Volume :
- 24
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 35001503
- Full Text :
- https://doi.org/10.1111/1462-2920.15891