Back to Search Start Over

Carriage of distinct bla KPC-2 and bla OXA-48 plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt.

Carriage of distinct bla KPC-2 and bla OXA-48 plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt.

Authors :
Yang Y
Yang Y
Ahmed MAEE
Qin M
He R
Wu Y
Liang X
Zhong LL
Chen P
Deng B
Hassan RM
Wen W
Xu L
Huang X
Xu L
Tian GB
Source :
BMC genomics [BMC Genomics] 2022 Jan 08; Vol. 23 (1), pp. 20. Date of Electronic Publication: 2022 Jan 08.
Publication Year :
2022

Abstract

Background: Carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) causes serious infections with significant morbidity and mortality. However, the epidemiology and transmission mechanisms of CR-hvKP and the corresponding carbapenem-resistant plasmids require further investigation. Herein, we have characterized an ST11 K. pneumoniae strain EBSI041 from the blood sample encoding both hypervirulence and carbapenem resistance phenotypes from a patient in Egypt.<br />Results: K. pneumoniae strain EBSI041 showed multidrug-resistance phenotypes, where it was highly resistant to almost all tested antibiotics including carbapenems. And hypervirulence phenotypes of EBSI041 was confirmed by the model of Galleria mellonella infection. Whole-genome sequencing analysis showed that the hybrid plasmid pEBSI041-1 carried a set of virulence factors rmpA, rmpA2, iucABCD and iutA, and six resistance genes aph(3')-VI, armA, msr(E), mph(E), qnrS, and sul2. Besides, bla <subscript>OXA-48</subscript> and bla <subscript>SHV-12</subscript> were harboured in a novel conjugative IncL-type plasmid pEBSI041-2. The bla <subscript>KPC-2</subscript> -carrying plasmid pEBSI041-3, a non-conjugative plasmid lacking the conjugative transfer genes, could be transferred with the help of pEBSI041-2, and the two plasmids could fuse into a new plasmid during co-transfer. Moreover, the emergence of the p16HN-263_KPC-like plasmids is likely due to the integration of pEBSI041-3 and pEBSI041-4 via IS26-mediated rearrangement.<br />Conclusion: To the best of our knowledge, this is the first report on the complete genome sequence of KPC-2- and OXA-48-coproducing hypervirulent K. pneumoniae from Egypt. These results give new insights into the adaptation and evolution of K. pneumoniae during nosocomial infections.<br /> (© 2021. The Author(s).)

Details

Language :
English
ISSN :
1471-2164
Volume :
23
Issue :
1
Database :
MEDLINE
Journal :
BMC genomics
Publication Type :
Academic Journal
Accession number :
34996351
Full Text :
https://doi.org/10.1186/s12864-021-08214-9