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Amoebicidal Activity of Poly-Epsilon-Lysine Functionalized Hydrogels.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2022 Jan 03; Vol. 63 (1), pp. 11. - Publication Year :
- 2022
-
Abstract
- Purpose: To determine the amoebicidal activity of functionalized poly-epsilon-lysine hydrogels (pɛK+) against Acanthamoeba castellanii.<br />Methods: A. castellanii trophozoites and cysts were grown in the presence of pɛK solution (0-2.17 mM), pɛK or pɛK+ hydrogels, or commercial hydrogel contact lens (CL) for 24 hours or 7 days in PBS or Peptone-Yeast-Glucose (PYG) media (nutrient-deplete or nutrient-replete cultures, respectively). Toxicity was determined using propidium iodide and imaged using fluorescence microscopy. Ex vivo porcine corneas were inoculated with A. castellanii trophozoites ± pɛK, pɛK+ hydrogels or commercial hydrogel CL for 7 days. Corneal infection was assessed by periodic acid-Schiff staining and histologic analysis. Regrowth of A. castellanii from hydrogel lenses and corneal discs at 7 days was assessed using microscopy and enumeration.<br />Results: The toxicity of pɛK+ hydrogels resulted in the death of 98.52% or 83.31% of the trophozoites at 24 hours or 7 days, respectively. The toxicity of pɛK+ hydrogels resulted in the death of 70.59% or 82.32% of the cysts in PBS at 24 hours or 7 days, respectively. Cysts exposed to pɛK+ hydrogels in PYG medium resulted in 75.37% and 87.14% death at 24 hours and 7 days. Ex vivo corneas infected with trophozoites and incubated with pɛK+ hydrogels showed the absence of A. castellanii in the stroma, with no regrowth from corneas or pɛK+ hydrogel, compared with infected-only corneas and those incubated in presence of commercial hydrogel CL.<br />Conclusions: pɛK+ hydrogels demonstrated pronounced amoebicidal and cysticidal activity against A. castellanii. pɛK+ hydrogels have the potential for use as CLs that could minimize the risk of CL-associated Acanthamoeba keratitis.
- Subjects :
- Acanthamoeba Keratitis parasitology
Amebicides toxicity
Animals
Cells, Cultured
Contact Lens Solutions pharmacology
Disease Models, Animal
Epithelium, Corneal drug effects
Eye Infections, Parasitic parasitology
Humans
Hydrogels toxicity
Microscopy, Fluorescence
Polylysine toxicity
Swine
Trophozoites drug effects
Acanthamoeba Keratitis drug therapy
Acanthamoeba castellanii drug effects
Amebicides pharmacology
Cornea parasitology
Eye Infections, Parasitic drug therapy
Hydrogels pharmacology
Polylysine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 63
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 34994769
- Full Text :
- https://doi.org/10.1167/iovs.63.1.11