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Effect of Twinlight Laser on the Attachment of Human Gingival Fibroblasts to the Root Surface In Vitro.
- Source :
-
Medical science monitor : international medical journal of experimental and clinical research [Med Sci Monit] 2022 Jan 05; Vol. 28, pp. e932191. Date of Electronic Publication: 2022 Jan 05. - Publication Year :
- 2022
-
Abstract
- BACKGROUND This study aimed to compare the effectiveness of subgingival scaling and root planing with the Twinlight laser, Er: YAG laser, and hand instrumentation on the removal of endotoxin and attachment of human gingival fibroblasts (HGFs) to cementum surfaces in vitro. MATERIAL AND METHODS Single-rooted teeth extracted for periodontal disease were collected and divided into 3 groups: group A, root planing with Gracey curet no. 5/6; group B, irradiation with Er: YAG laser; group C, irradiation with Er: YAG laser and Nd: YAG laser. Endotoxins were determined by the limulus amebocyte lysate test. Cell attachment and proliferation of HGFs on root specimens were evaluated by cell counting kit-8 assay. The root surface and cell morphology were observed by scanning electron microscope. RESULTS A flat root surface with scratches was found in group A, Group B had a homogeneous rough morphology without carbonization, and group C had a non-homogeneous rough morphology with ablation. The endotoxin concentration was highest in group A (P<0.05) and lowest in group C (P>0.05). HGFs cultured in group B showed significantly increased adhesion and proliferation compared with groups A and C (P<0.05). HGFs in group B were well attached, covered densely by pseudopodia. HGFs in group A were round with poor extension and short pseudopodia, while the cells in the group C were in narrow, triangular, or polygonal shapes. CONCLUSIONS Twinlight laser-assisted periodontal treatment effectively improved the biocompatibility of root surface and promoted the attachment and proliferation of fibroblasts by removing calculus and reducing the concentration of endotoxins.
- Subjects :
- Cell Adhesion
Humans
Microscopy, Electron, Scanning methods
Surface Properties
Fibroblasts physiology
Gingiva microbiology
Gingiva pathology
Laser Therapy instrumentation
Laser Therapy methods
Lasers, Solid-State therapeutic use
Periodontal Diseases microbiology
Periodontal Diseases physiopathology
Periodontal Diseases therapy
Root Planing methods
Subjects
Details
- Language :
- English
- ISSN :
- 1643-3750
- Volume :
- 28
- Database :
- MEDLINE
- Journal :
- Medical science monitor : international medical journal of experimental and clinical research
- Publication Type :
- Academic Journal
- Accession number :
- 34983919
- Full Text :
- https://doi.org/10.12659/MSM.932191