Calculating Half Maximal Inhibitory Concentration (IC 50 ) Values from Glycomics Microarray Data Using GraphPad Prism.

Authors :: Le Berre M
Gerlach JQ
Dziembała I
Kilcoyne M
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2460, pp. 89-111.
Publication Year :: 2022
Abstract: Half maximal inhibitory concentration (IC <subscript>50</subscript> ) is a measurement often used to compare the efficiency of various carbohydrates and their derivatives for inhibition of lectin binding to particular ligands. IC <subscript>50</subscript> values can be calculated using experimental data from various platforms including enzyme-linked immunosorbent assay- (ELISA-)type microtiter plate assays, isothermal titration calorimetry (ITC), or glycan microarrays. In this chapter, we describe methods to fluorescently label a lectin, to carry out a lectin binding inhibition experiment on glycan microarrays, and to calculate the IC <subscript>50</subscript> value of a binding inhibitory molecule using GraphPad Prism software. In the example used to illustrate the method in this chapter, IC <subscript>50</subscript> calculation is demonstrated for inhibition of Maackia amurensis agglutinin (MAA) binding to 3'sialyl-N-acetyllactosamine (3SLN) using free lactose.<br /> (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)

Subjects :: Carbohydrates chemistry
Microarray Analysis
Polysaccharides chemistry
Glycomics
Lectins metabolism

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