Near-Infrared Mitochondria-Targetable Single-Molecule probe for Dual-Response of viscosity and sulfur dioxide in vivo.

Studies have demonstrated that the viscosity and SO ₂ in mitochondria are involved in various physiological processes, which are both important for maintaining the normal function of mitochondria. In this research, we rationally designed and synthesized a novel mitochondria-targetable fluorescent probe Mito-MG with near-infrared absorption and emission for dual-response of viscosity and SO ₂ . Based on the TICT mechanism and Michael addition reaction, the probe Mito-MG responds to viscosity and SO ₂ with different near-infrared fluorescence signals. Importantly, Mito-MG presented outstanding mitochondrial targeting ability (Pearson's colocalization coefficient is 0.8602) and has been successfully applied to imaging of the cells, zebrafish and mice. We believe that Mito-MG could be applied as a powerful tool for a deep understanding of the connection of viscosity and SO ₂ in biological systems.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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