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MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria.

Authors :
Zheng ZJ
Cui ZH
Diao QY
Ye XQ
Zhong ZX
Tang T
Wu SB
He HL
Lian XL
Fang LX
Wang XR
Liang LJ
Liu YH
Liao XP
Sun J
Source :
Virulence [Virulence] 2022 Dec; Vol. 13 (1), pp. 77-88.
Publication Year :
2022

Abstract

The extensive use of tetracycline antibiotics has led to the widespread presence of tetracycline-resistance genes in Gram-negative bacteria and this poses serious threats to human and animal health. In our previous study, we reported a method for rapid detection of Tet(X)-producers using MALDI-TOF MS. However, there have been multiple machineries involved in tetracycline resistance including efflux pump, and ribosomal protection protein. Our previous demonstrated the limitation in probing the non-Tet(X)-producing tetracycline-resistant strains. In this regard, we further developed a MALDI-TOF MS method to detect and differentiate Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant strains. Test strains were incubated with tigecycline and oxytetracycline in separate tubes for 3 h and then analyzed spectral peaks of tigecycline, oxytetracycline, and their metabolite. Strains were distinguished using MS ratio for [metabolite/(metabolite+ tigecycline or oxytetracycline)]. Four control strains and 319 test strains were analyzed and the sensitivity was 98.90% and specificity was 98.34%. This was consistent with the results obtained from LC-MS/MS analysis. Interestingly, we also found that the reactive oxygen species (ROS) produced by tetracycline-susceptible strains were able to promote the degradation of oxytetracycline. Overall, the MALDI <superscript>Tet(X)-plus</superscript> test represents a rapid and reliable method to detect Tet(X)-producers, non-Tet(X)-producing tetracycline-resistant strains, and tetracycline-susceptible strains.

Details

Language :
English
ISSN :
2150-5608
Volume :
13
Issue :
1
Database :
MEDLINE
Journal :
Virulence
Publication Type :
Academic Journal
Accession number :
34951562
Full Text :
https://doi.org/10.1080/21505594.2021.2018768