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Continuous reference intervals for pediatric testosterone, sex hormone binding globulin and free testosterone using quantile regression.

Authors :
Holmes DT
van der Gugten JG
Jung B
McCudden CR
Source :
Journal of mass spectrometry and advances in the clinical lab [J Mass Spectrom Adv Clin Lab] 2021 Nov 01; Vol. 22, pp. 64-70. Date of Electronic Publication: 2021 Nov 01 (Print Publication: 2021).
Publication Year :
2021

Abstract

Testosterone (T), sex hormone binding globulin (SHBG), free testosterone (FT) and bioavailable testosterone (BAT) are commonly employed tests in pediatric endocrinology and all require age-dependent reference intervals for interpretation. The common methods used to derive these reference intervals require decisions about data shape and/or age partition thresholds, which can result in sharp differences between age groups, particularly for pubescent children. Partitioning also results in a form of data loss, where data from one age-bin is completed disconnected from the adjacent age-bins. Non-parametric continuous reference intervals methods have previously been developed to avoid some of these drawbacks. These strategies use all the available data and smooth transitions between ages avoiding partitioning. However, the fitting process involves selection and adjustment of many parameters and it can be difficult to maintain a reproducible approach. Here we provide a workflow for non-parametric continuous reference intervals applied to T, FT, BAT, and SHBG using the R language quantregGrowth package. T measurements were determined by LC-MS/MS, FT and BAT were calculated, and SHBG was measured on the Roche Cobas e601. The continuous interval methodology is described in detail with code examples and illustrations for reproducibility.<br /> (©2021THEAUTHORS.PublishingservicesbyELSEVIERB.V.)

Details

Language :
English
ISSN :
2667-145X
Volume :
22
Database :
MEDLINE
Journal :
Journal of mass spectrometry and advances in the clinical lab
Publication Type :
Academic Journal
Accession number :
34939057
Full Text :
https://doi.org/10.1016/j.jmsacl.2021.10.005