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[SP1 identified as a transcription factor of miR-122-5p].
- Source :
-
Zhonghua nan ke xue = National journal of andrology [Zhonghua Nan Ke Xue] 2021 Jan; Vol. 27 (1), pp. 11-16. - Publication Year :
- 2021
-
Abstract
- Objective: To study the transcription factors of the spermatogenesis-related promoter mir-122-5p.<br />Methods: SP1 and GATA4 were predicted as the possible transcription factors of the mir-122-5p promoter by bioinformatics analysis, followed by construction of the double luciferase pGL3-mir-122-5p promoter vector, pcDNA3.1 (+) -SP1 expression vector and pcDNA3.1 (+) -GATA4 expression vector, respectively. The pcDNA-SP1+pGL3-basic mixture plasmid and pcDNA-SP1+ pGL3-miR-122-5p promoter mixture plasmid, pcDNA-GATA4+pGL3-basic mixture plasmid and pcDNA-GATA4+pGL3-miR-122-5p promoter mixture plasmid were transferred into 293T cells. The enzyme activity was detected the Dual-Luciferase Reporter Assay System.<br />Results: The fluorescence value of the pcDNA3.1+pGL3-miR-122 promoter was 0.0362 ± 0.0004, significantly higher than that of the pcDNA3.1+pGL3-basic group (P < 0.05), indicating the successful construction of the mouse miR-122-5p promoter luciferase reporter plasmid. The fluorescence value was markedly higher in the pcDNA -SP1 + pGL3-miR-122-5p promoter than in the pcDNA -SP1+pGL3-basic group, suggesting that the transcription factor SP1 could promote the transcription of miR-122. There was no statistically significant difference in the fluorescence value between the pcDNA -gata4+pGL3-basic transfection and pcDNA -GATA4+pGL3-miR-122-5p promoter transfection groups, indicative of the inability of GATA4 to promote the transcription of miR-122-5p.<br />Conclusions: The transcription factor SP1, rather than GATA4, can promote the transcription of miR-122-5p.
- Subjects :
- Animals
Mice
MicroRNAs genetics
Transcription Factors genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1009-3591
- Volume :
- 27
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhonghua nan ke xue = National journal of andrology
- Publication Type :
- Academic Journal
- Accession number :
- 34914275