Suggested Cutoff Point for Testosterone by Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) after Stimulation with Recombinant Human Chorionic Gonadotropin.

Authors :: de Oliveira LR
Longui CA
Guaragna-Filho G
da Costa JL
Lanaro R
Chiamolera MI
de Mello MP
Morcillo AM
Maciel-Guerra AT
Guerra-Junior G
Source :: Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation [Sex Dev] 2022; Vol. 16 (4), pp. 266-269. Date of Electronic Publication: 2021 Dec 14.
Publication Year :: 2022
Abstract: The human chorionic gonadotropin (hCG) stimulation test that evaluates gonadal steroidogenesis is crucial in the assessment of patients with 46,XY disorders of sex development (DSD). This study aimed to determine a testosterone (T) cutoff level that indicates an adequate testicular function using LC-MS/MS after stimulation with recombinant human chorionic gonadotropin (rhCG) in a single dose. Nineteen prepubertal children with 46,XY DSD and normal T secretion were evaluated. T and dihydrotestosterone (DHT) levels were measured by liquid chromatography technique with tandem mass spectrometry (LC-MS/MS) before and 7 days after rhCG application at 250 µg. We suggest 0.89 ng/mL as the cutoff point for T after rhCG stimulation analyzed by LC-MS/MS.<br /> (© 2021 S. Karger AG, Basel.)

Subjects :: Child
Humans
Chromatography, Liquid
Chorionic Gonadotropin pharmacology
Testosterone pharmacology
Tandem Mass Spectrometry

Language :: English
ISSN :: 1661-5433
Volume :: 16
Issue :: 4
Database :: MEDLINE
Journal :: Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation
Publication Type :: Academic Journal
Accession number :: 34903701
Full Text :: https://doi.org/10.1159/000519422

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