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A Wheat TaTOE1-B1 Transcript TaTOE1-B1-3 Can Delay the Flowering Time of Transgenic Arabidopsis .
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 23; Vol. 22 (23). Date of Electronic Publication: 2021 Nov 23. - Publication Year :
- 2021
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Abstract
- Flowering time is one of the most important agronomic traits in wheat production. A proper flowering time might contribute to the reduction or avoidance of biotic and abiotic stresses, adjust plant architecture, and affect the yield and quality of grain. In this study, TaTOE1-B1 in wheat produced three transcripts ( TaTOE1-B1-1 , TaTOE1-B1-2 , and TaTOE1-B1-3 ) by alternative splicing. Compared to the longest transcript, TaTOE1-B1-1 , TaTOE1-B1-3 has a deletion in the sixth exon (1219-1264 bp). Under long-day conditions, the heterologous overexpression of the TaTOE1-B1-3 gene delayed flowering, prolonged the vegetative growth time, and enlarged the vegetative body of Arabidopsis , but that of TaTOE1-B1-1 did not. As typical AP2 family members, TaTOE1-B1-1 and TaTOE1-B1-3 are mainly located in the nucleus and have transcriptional activation activities; the transcriptional activation region of TaTOE1-B1-3 is located in the C-terminal. In TaTOE1-B1-3 overexpression lines, the expression of flowering-related AtFT and AtSOC1 genes is significantly downregulated. In addition, this study confirms the protein-protein interaction between TaTOE1-B1-3 and TaPIFI, which may play an important role in flowering inhibition. These results provide a theoretical basis for the precise regulation of wheat flowering time.
- Subjects :
- Alternative Splicing
Arabidopsis genetics
Cloning, Molecular
Computational Biology
Evolution, Molecular
Flowers genetics
Flowers physiology
Gene Expression Regulation, Plant
Plant Proteins genetics
Plant Proteins metabolism
Plants, Genetically Modified physiology
Transcription Factors metabolism
Triticum genetics
Arabidopsis physiology
Transcription Factors genetics
Triticum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34884449
- Full Text :
- https://doi.org/10.3390/ijms222312645