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Detection of Neisseria meningitidis in saliva and oropharyngeal samples from college students.
- Source :
-
Scientific reports [Sci Rep] 2021 Nov 30; Vol. 11 (1), pp. 23138. Date of Electronic Publication: 2021 Nov 30. - Publication Year :
- 2021
-
Abstract
- Carriage of Neisseria meningitidis is an accepted endpoint in monitoring meningococcal vaccines effects. We have assessed N. meningitidis and vaccine-type genogroup carriage prevalence in college students at the time of MenACWY vaccine introduction in the Netherlands, and evaluated the feasibility of saliva sampling for the surveillance of carriage. For this, paired saliva and oropharyngeal samples collected from 299 students were cultured for meningococcus. The DNA extracted from all bacterial growth was subjected to qPCRs quantifying meningococcal and genogroup-specific genes presence. Samples negative by culture yet positive for qPCR were cultured again for meningococcus. Altogether 74 (25%) of students were identified as meningococcal carrier by any method. Sixty-one students (20%) were identified as carriers with qPCR. The difference between number of qPCR-positive oropharyngeal (n = 59) and saliva (n = 52) samples was not significant (McNemar's test, p = 0.07). Meningococci were cultured from 72 students (24%), with a significantly higher (p < 0.001) number of oropharyngeal (n = 70) compared with saliva (n = 54) samples. The prevalence of genogroups A, B, C, W, and Y was none, 9%, 1%, 1% and 6%, respectively, and 8% of students carried MenACWY vaccine-type genogroup meningococci. Saliva is easy to collect and when combined with qPCR detection can be considered for meningococcal carriage studies.<br /> (© 2021. The Author(s).)
- Subjects :
- Adolescent
Adult
Carrier State microbiology
Cross-Sectional Studies
Female
Genotype
Humans
Male
Meningococcal Vaccines
Netherlands
Prevalence
Risk Factors
Students
Vaccines, Conjugate
Young Adult
Meningococcal Infections diagnosis
Meningococcal Infections microbiology
Neisseria meningitidis genetics
Neisseria meningitidis, Serogroup B genetics
Oropharynx metabolism
Saliva microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 34848796
- Full Text :
- https://doi.org/10.1038/s41598-021-02555-x