Back to Search
Start Over
A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2316, pp. 89-96. - Publication Year :
- 2022
-
Abstract
- A simplified dot-blot hybridization protocol for Potato spindle tuber viroid (PSTVd) detection in Solanaceae species is described here. The protocol uses an RNA DIG-labeled probe and a simplified extraction procedure that avoids the use of hazardous chemicals. PSTVd was detected in composite tomato leaf samples in a ratio of up to 1:15 of PSTVd-infected to non-infected tissue and in composite potato tuber samples in a ratio up to 1:5 of PSTVd-infected to non-infected tissue. In Brugmansia spp., PSTVd was detected solely in the standard sample extract preparation. The method is suitable for a reliable, large-scale sample screening especially where cost is a limiting factor.<br /> (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2316
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34845688
- Full Text :
- https://doi.org/10.1007/978-1-0716-1464-8_9