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The microbial composition of the initial insult can predict the prognosis of experimental sepsis.
- Source :
-
Scientific reports [Sci Rep] 2021 Nov 23; Vol. 11 (1), pp. 22772. Date of Electronic Publication: 2021 Nov 23. - Publication Year :
- 2021
-
Abstract
- We hypothesized that the composition of sepsis-inducing bacterial flora influences the course of fecal peritonitis in rodents. Saline or fecal suspensions with a standardized dose range of bacterial colony-forming units (CFUs) were injected intraperitoneally into Sprague-Dawley rats. The qualitative composition of the initial inoculum and the ascites was analyzed separately by MALDI-TOF mass spectrometry. Invasive monitoring was conducted in separate anesthetized groups (n = 12-13/group) after 12, 24, 48 and 72 h to determine rat-specific organ failure assessment (ROFA) scores. Death and ROFA scores peaked at 24 h. At this time, 20% mortality occurred in animals receiving a monomicrobial E. coli suspension, and ROFA scores were significantly higher in the monomicrobial subgroup than in the polymicrobial one (median 6.5; 5.0-7.0 and 5.0; 4.75-5.0, respectively). ROFA scores dropped after 48 h, accompanied by a steady decrease in ascites CFUs and a shift towards intra-abdominal monomicrobial E. coli cultures. Furthermore, we found a relationship between ascites CFUs and the evolving change in ROFA scores throughout the study. Hence, quantitatively identical bacterial loads with mono- or polymicrobial dominance lead to a different degree of sepsis severity and divergent outcomes. Initial and intraperitoneal microbiological testing should be used to improve translational research success.<br /> (© 2021. The Author(s).)
- Subjects :
- Animals
Bacteria isolation & purification
Bacterial Infections microbiology
Male
Multiple Organ Failure etiology
Prognosis
Rats
Rats, Sprague-Dawley
Sepsis etiology
Sepsis microbiology
Bacteria classification
Bacteria pathogenicity
Bacterial Infections complications
Disease Models, Animal
Multiple Organ Failure pathology
Sepsis pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 34815465
- Full Text :
- https://doi.org/10.1038/s41598-021-02129-x