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Phage-Based Profiling of Rare Single Cells Using Nanoparticle-Directed Capture.
- Source :
-
ACS nano [ACS Nano] 2021 Dec 28; Vol. 15 (12), pp. 19202-19210. Date of Electronic Publication: 2021 Nov 23. - Publication Year :
- 2021
-
Abstract
- Advances in single-cell level profiling of the proteome require quantitative and versatile platforms, especially for rare cell analyses such as circulating tumor cell (CTC) profiling. Here we demonstrate an integrated microfluidic chip that uses magnetic nanoparticles to capture single tumor cells with high efficiency, permits on-chip incubation, and facilitates in situ cell-surface protein expression analysis. Combined with phage-based barcoding and next-generation sequencing technology, we were able to monitor changes in the expression of multiple surface markers stimulated in response to CTC adherence. Interestingly, we found fluctuations in the expression of Frizzled2 (FZD2) that reflected the microenvironment of the single cells. This platform has a high potential for in-depth screening of multiple surface antigens simultaneously in rare cells with single-cell resolution, which will provide further insights regarding biological heterogeneity and human disease.
Details
- Language :
- English
- ISSN :
- 1936-086X
- Volume :
- 15
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- ACS nano
- Publication Type :
- Academic Journal
- Accession number :
- 34813293
- Full Text :
- https://doi.org/10.1021/acsnano.1c03935