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Effects of allyl isothiocyanate on the expression, function, and its mechanism of ABCA1 and ABCG1 in pulmonary of COPD rats.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2021 Dec; Vol. 101 (Pt B), pp. 108373. Date of Electronic Publication: 2021 Nov 19. - Publication Year :
- 2021
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Abstract
- Background and Aims: Allyl isothiocyanate(AITC) has been shown to play an important role in the improved symptoms of chronic obstructive pulmonary disease(COPD) and the inhibition of inflammation, but the role in COPD lipid metabolism disorder and the molecular mechanism remains unclear. We aimed to explore whether and how AITC affects COPD by regulating lipid metabolism and inflammatory response.<br />Methods: The COPD rat model was established by cigarette smoke exposure. Cigarette smoke extract stimulated 16HBE cells to induce a cell model. The effect of AITC treatment was detected by lung function test, H&E staining, Oil red O staining, immunohistochemistry, ELISA, CCK-8, HPLC, fluorescence efflux test, siRNA, RT-PCR, and Western blotting. Biological analysis was performed to analyze the results. Graphpad Prism 8.0 software was used for statistical analysis.<br />Results: AITC can improve lung function and pathological injury in COPD rats. The levels of IL-1 β and TNF- α in the AITC treatment group were significantly lower than those in the model group(P < 0.05), and the lipid metabolism was also improved (P < 0.05). AITC reverses CSE-induced down-regulation of LXR α, ABCA1, and ABCG1 expression and function in a time-and concentration-dependent manner (P < 0.05). AITC regulates the cholesterol metabolism disorder induced by CSE in NR8383 cells and attenuates macrophage inflammation (P < 0.05). In addition, after silencing LXR α with siRNA, the effect of AITC was also inhibited.<br />Conclusion: These results suggest that AITC improves COPD by promoting RCT process and reducing inflammatory response via activating LXR pathways.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- ATP Binding Cassette Transporter 1 genetics
ATP Binding Cassette Transporter, Subfamily G, Member 1 genetics
Animals
Cell Line
Food Preservatives pharmacology
Liver X Receptors agonists
Lung drug effects
Lung metabolism
Male
Pulmonary Disease, Chronic Obstructive metabolism
Rats
Rats, Sprague-Dawley
Up-Regulation
ATP Binding Cassette Transporter 1 metabolism
ATP Binding Cassette Transporter, Subfamily G, Member 1 metabolism
Gene Expression Regulation drug effects
Isothiocyanates pharmacology
Pulmonary Disease, Chronic Obstructive chemically induced
Smoke adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 101
- Issue :
- Pt B
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 34802946
- Full Text :
- https://doi.org/10.1016/j.intimp.2021.108373