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The potential role of hsa_circ_0001079 in androgenetic alopecia via sponging hsa-miR-136-5p.
- Source :
-
Journal of clinical laboratory analysis [J Clin Lab Anal] 2022 Jan; Vol. 36 (1), pp. e24021. Date of Electronic Publication: 2021 Nov 17. - Publication Year :
- 2022
-
Abstract
- Background: Androgenetic alopecia (AGA) is an androgen-dependent polygenic hereditary disease.<br />Methods: Diseased hair follicles from 5 AGA patients and normal hair follicles from 5 healthy individuals were selected as specimens to carry out whole transcriptome sequencing. Multiple high-expression circular RNAs (circRNAs) were screened from the diseased group. We further verified the presence of the circRNAs in the clinical specimens by real-time fluorescence quantitative PCR (qRT-PCR).<br />Results: In total, 100 circRNAs were significantly upregulated, and 184 circRNAs were significantly downregulated. The top 10 upregulated circRNAs were hsa&#95;circ&#95;0101041, hsa&#95;circ&#95;0001578, hsa&#95;circ&#95;0135062, hsa&#95;circ&#95;0002980, hsa&#95;circ&#95;0005062, hsa&#95;circ&#95;0072688, hsa&#95;circ&#95;0133954, hsa&#95;circ&#95;0001079, hsa&#95;circ&#95;0005974, hsa&#95;circ&#95;0000489. The top 10 downregulated circRNAs were hsa&#95;circ&#95;0001278, hsa&#95;circ&#95;0031482, hsa&#95;circ&#95;0008285, hsa&#95;circ&#95;0003784, hsa&#95;circ&#95;0077096, hsa&#95;circ&#95;0001148, hsa&#95;circ&#95;0006886, hsa&#95;circ&#95;0000638, hsa&#95;circ&#95;0084521, and hsa&#95;circ&#95;0101074. Among top 10 upregulated circRNAs, hsa&#95;circ&#95;0001079 showed significantly high expression via large-sample verification and clinical application potential. Based on a database comparison and base pairing analysis, we found that has-miR-136-5p bound to hsa&#95;circ&#95;0001079 and that hsa-miR-136-5p had potential binding sites with Wnt5A.<br />Conclusion: In summary, through high-throughput sequencing and bioinformatic analysis, a potential diagnostic marker for alopecia and a key circRNA that might adsorb microRNA (miRNA) through a sponging mechanism, thus mediating alopecia, were discovered in this study.<br /> (© 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.)
- Subjects :
- Adult
Biomarkers metabolism
Computational Biology
Hair Follicle chemistry
Hair Follicle metabolism
High-Throughput Nucleotide Sequencing
Humans
Male
MicroRNAs metabolism
RNA, Circular analysis
RNA, Circular metabolism
Transcriptome genetics
Wnt-5a Protein genetics
Wnt-5a Protein metabolism
Alopecia genetics
Alopecia metabolism
Alopecia pathology
MicroRNAs genetics
RNA, Circular genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-2825
- Volume :
- 36
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of clinical laboratory analysis
- Publication Type :
- Academic Journal
- Accession number :
- 34788492
- Full Text :
- https://doi.org/10.1002/jcla.24021