Back to Search
Start Over
Chromatin spatial organization of wild type and mutant peanuts reveals high-resolution genomic architecture and interaction alterations.
- Source :
-
Genome biology [Genome Biol] 2021 Nov 16; Vol. 22 (1), pp. 315. Date of Electronic Publication: 2021 Nov 16. - Publication Year :
- 2021
-
Abstract
- Background: Three-dimensional (3D) chromatin organization provides a critical foundation to investigate gene expression regulation and cellular homeostasis.<br />Results: Here, we present the first 3D genome architecture maps in wild type and mutant allotetraploid peanut lines, which illustrate A/B compartments, topologically associated domains (TADs), and widespread chromatin interactions. Most peanut chromosomal arms (52.3%) have active regions (A compartments) with relatively high gene density and high transcriptional levels. About 2.0% of chromosomal regions switch from inactive to active (B-to-A) in the mutant line, harboring 58 differentially expressed genes enriched in flavonoid biosynthesis and circadian rhythm functions. The mutant peanut line shows a higher number of genome-wide cis-interactions than its wild-type. The present study reveals a new TAD in the mutant line that generates different chromatin loops and harbors a specific upstream AP2EREBP-binding motif which might upregulate the expression of the GA2ox gene and decrease active gibberellin (GA) content, presumably making the mutant plant dwarf.<br />Conclusions: Our findings will shed new light on the relationship between 3D chromatin architecture and transcriptional regulation in plants.<br /> (© 2021. The Author(s).)
Details
- Language :
- English
- ISSN :
- 1474-760X
- Volume :
- 22
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genome biology
- Publication Type :
- Academic Journal
- Accession number :
- 34784945
- Full Text :
- https://doi.org/10.1186/s13059-021-02520-x