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Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat.
- Source :
-
BMC urology [BMC Urol] 2021 Nov 13; Vol. 21 (1), pp. 156. Date of Electronic Publication: 2021 Nov 13. - Publication Year :
- 2021
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Abstract
- Background: Interstitial cystitis/bladder pain syndrome (IC/BPS) categorized with and without Hunner lesions is a condition that displays chronic pelvic pain related to the bladder with no efficacious treatment options. There are strong associations suggested between Hunner-type IC and autoimmune diseases. Recently, we established an animal model of Hunner-type IC using a Toll-like receptor-7 (TLR7) agonist. Intravenous infusion of mesenchymal stem cells (MSCs) can be used to treat injury via multimodal and orchestrated therapeutic mechanisms including anti-inflammatory effects. Here, we investigated whether infused MSCs elicit therapeutic efficacy associated with the TLR7-related anti-inflammatory pathway in our Hunner-type IC model.<br />Methods: Voiding behaviors were monitored 24 h prior to the Loxoribine (LX), which is a TLR7 agonist instillation in order to establish a Hunner-type IC model (from - 24 to 0 h) in female Sprague-Dawley rats. LX was instilled transurethrally into the bladder. At 0 h, the initial freezing behavior test confirmed that no freezing behavior was observed in any of the animals. The LX-instilled animals were randomized. Randomized LX-instilled rats were intravenously infused with MSCs or with vehicle through the right external jugular vein. Sampling tissue for green fluorescent protein (GFP)-positive MSCs were carried out at 48 h. Second voiding behavior tests were monitored from 72 to 96 h. After the final evaluation of the freezing behavior test at 96 h after LX instillation (72 h after MSC or vehicle infusion), histological evaluation with H&E staining and quantitative real-time polymerase chain reaction (RT-PCR) to analyze the mRNA expression levels of inflammatory cytokines were performed.<br />Results: Freezing behavior was reduced in the MSC group, and voiding behavior in the MSC group did not deteriorate. Hematoxylin-eosin staining showed that mucosal edema, leukocyte infiltration, and hemorrhage were suppressed in the MSC group. The relative expression of interferon-β mRNA in the bladder of the MSC group was inhibited. Numerous GFP-positive MSCs were distributed mainly in the submucosal and mucosal layers of the inflammatory bladder wall.<br />Conclusion: Intravenous infusion of MSCs may have therapeutic efficacy in a LX-instilled Hunner-type IC rat model via a TLR7-related anti-inflammatory pathway.<br /> (© 2021. The Author(s).)
- Subjects :
- Animals
Behavior, Animal
Cystitis, Interstitial chemically induced
Cystitis, Interstitial metabolism
Cystitis, Interstitial pathology
Disease Models, Animal
Down-Regulation
Female
Infusions, Intravenous
Pelvic Pain etiology
Rats
Rats, Sprague-Dawley
Urinary Bladder pathology
Urination
Cystitis, Interstitial therapy
Interferon-beta metabolism
Mesenchymal Stem Cells
Toll-Like Receptor 7 agonists
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2490
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC urology
- Publication Type :
- Academic Journal
- Accession number :
- 34774029
- Full Text :
- https://doi.org/10.1186/s12894-021-00923-3