Back to Search
Start Over
FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot.
- Source :
-
PLoS biology [PLoS Biol] 2021 Nov 12; Vol. 19 (11), pp. e3001454. Date of Electronic Publication: 2021 Nov 12 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell's response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Arabidopsis cytology
Arabidopsis drug effects
Arabidopsis Proteins genetics
Benzamides pharmacology
Biomechanical Phenomena
Hypocotyl anatomy & histology
Hypocotyl drug effects
Microtubules drug effects
Mutation genetics
Phenotype
Phosphotransferases genetics
Plant Shoots cytology
Stress, Mechanical
Tensile Strength
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Microtubules metabolism
Phosphotransferases metabolism
Plant Shoots physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1545-7885
- Volume :
- 19
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PLoS biology
- Publication Type :
- Academic Journal
- Accession number :
- 34767544
- Full Text :
- https://doi.org/10.1371/journal.pbio.3001454