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DNA methylation-mediated down-regulation of TMEM130 promotes cell migration in breast cancer.
- Source :
-
Acta histochemica [Acta Histochem] 2021 Dec; Vol. 123 (8), pp. 151814. Date of Electronic Publication: 2021 Nov 08. - Publication Year :
- 2021
-
Abstract
- Background: Breast cancer is the most common female cancer worldwide. DNA methylation is a common modification in epigenetics and affects the prognosis of breast cancer by changing gene expression. In the present study, we aim to investigate the role of DNA methylation in TMEM130 gene expression, and the function of TMEM130 in breast cancer cell migration.<br />Methods: The transcriptional expression of TMEM130 was detected by qRT-PCR in breast cancer cell lines and tissues. Bisulfite sequencing PCR (BSP) was used to confirm the methylation status of TMEM130 promoter. Then, TMEM130 was transfected in breast cancer cell lines and to explore its role in cell migration by Transwell and western blot.<br />Results: TMEM130 mRNA expression was decreased in breast cancer cell lines and tissues, and consistent with the data in The Cancer Genome Atlas (TCGA). The promoter of TMEM130 was hypermethylated in breast cancer and the expression of TMEM130 could be restored by the methyltransferase inhibitor. Overexpression of TMEM130 could inhibit cell migration ability in breast cancer cell lines.<br />Conclusion: Taken together, these results indicate TMEM130 downregulation and hypermethylation might contribute to breast cancer migration and TMEM130 might be a promising biomarker for breast cancer.<br /> (Copyright © 2021 Elsevier GmbH. All rights reserved.)
- Subjects :
- Biomarkers, Tumor genetics
Breast Neoplasms genetics
DNA, Neoplasm genetics
Female
Humans
MCF-7 Cells
Membrane Proteins genetics
Neoplasm Proteins genetics
Biomarkers, Tumor biosynthesis
Breast Neoplasms metabolism
DNA Methylation
DNA, Neoplasm metabolism
Down-Regulation
Gene Expression Regulation, Neoplastic
Membrane Proteins biosynthesis
Neoplasm Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0372
- Volume :
- 123
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Acta histochemica
- Publication Type :
- Academic Journal
- Accession number :
- 34763116
- Full Text :
- https://doi.org/10.1016/j.acthis.2021.151814