Pyrophosphate and Irreversibility in Evolution, or why PP i Is Not an Energy Currency and why Nature Chose Triphosphates.

The possible evolutionary significance of pyrophosphate (PP _i ) has been discussed since the early 1960s. Lipmann suggested that PP _i could have been an ancient currency or a possible environmental source of metabolic energy at origins, while Kornberg proposed that PP _i vectorializes metabolism because ubiquitous pyrophosphatases render PP _i forming reactions kinetically irreversible. To test those ideas, we investigated the reactions that consume phosphoanhydride bonds among the 402 reactions of the universal biosynthetic core that generates amino acids, nucleotides, and cofactors from H ₂ , CO ₂ , and NH ₃ . We find that 36% of the core's phosphoanhydride hydrolyzing reactions generate PP _i , while no reactions use PP _i as an energy currency. The polymerization reactions that generate ~80% of cell mass - protein, RNA, and DNA synthesis - all generate PP _i , while none use PP _i as an energy source. In typical prokaryotic cells, aminoacyl tRNA synthetases (AARS) underlie ~80% of PP _i production. We show that the irreversibility of the AARS reaction is a kinetic, not a thermodynamic effect. The data indicate that PP _i is not an ancient energy currency and probably never was. Instead, PP _i hydrolysis is an ancient mechanism that imparts irreversibility, as Kornberg suggested, functioning like a ratchet's pawl to vectorialize the life process toward growth. The two anhydride bonds in nucleoside triphosphates offer ATP-cleaving enzymes an option to impart either thermodynamic control (P _i formation) or kinetic control (PP _i formation) upon reactions. This dual capacity explains why nature chose the triphosphate moiety of ATP as biochemistry's universal energy currency.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Wimmer, Kleinermanns and Martin.)