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Identification and Evaluation of Recombinant Outer Membrane Proteins as Vaccine Candidates Against Klebsiella pneumoniae .
- Source :
-
Frontiers in immunology [Front Immunol] 2021 Oct 20; Vol. 12, pp. 730116. Date of Electronic Publication: 2021 Oct 20 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Klebsiella pneumoniae found in the normal flora of the human oral and intestinal tract mainly causes hospital-acquired infections but can also cause community-acquired infections. To date, most clinical trials of vaccines against K. pneumoniae have ended in failure. Furthermore, no single conserved protein has been identified as an antigen candidate to accelerate vaccine development. In this study, we identified five outer membrane proteins of K. pneumoniae , namely, Kpn&#95;Omp001, Kpn&#95;Omp002, Kpn&#95;Omp003, Kpn&#95;Omp004, and Kpn&#95;Omp005, by using reliable second-generation proteomics and bioinformatics. Mice vaccinated with these five KOMPs elicited significantly higher antigen-specific IgG, IgG1, and IgG2a. However, only Kpn&#95;Omp001, Kpn&#95;Omp002, and Kpn&#95;Omp005 were able to induce a protective immune response with two K. pneumoniae infection models. These protective effects were accompanied by the involvement of different immune responses induced by KOMPs, which included KOMPs-specific IFN-γ-, IL4-, and IL17A-mediated immune responses. These findings indicate that Kpn&#95;Omp001, Kpn&#95;Omp002, and Kpn&#95;Omp005 are three potential Th1, Th2, and Th17 candidate antigens, which could be developed into multivalent and serotype-independent vaccines against K. pneumoniae infection.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2021 Zhang, Hu, Dou, Xiong, Wang, Hu, Xing, Li, Cai, Jin, Zhang, Lin, Li, Yuen and Huang.)
- Subjects :
- Animals
Bacterial Load
Bacterial Outer Membrane Proteins genetics
Bacterial Outer Membrane Proteins immunology
Bacterial Vaccines genetics
Bacterial Vaccines immunology
Disease Models, Animal
HL-60 Cells
Humans
Immunogenicity, Vaccine
Klebsiella Infections immunology
Klebsiella Infections microbiology
Klebsiella pneumoniae genetics
Mice, Inbred BALB C
Phagocytes immunology
Phagocytes microbiology
Phagocytosis
Recombinant Proteins immunology
Recombinant Proteins pharmacology
T-Lymphocytes immunology
T-Lymphocytes microbiology
Vaccination
Vaccines, Synthetic immunology
Vaccines, Synthetic pharmacology
Mice
Bacterial Outer Membrane Proteins pharmacology
Bacterial Vaccines pharmacology
Klebsiella Infections prevention & control
Klebsiella pneumoniae immunology
Vaccine Development
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 34745099
- Full Text :
- https://doi.org/10.3389/fimmu.2021.730116