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Salidroside orchestrates metabolic reprogramming by regulating the Hif-1α signalling pathway in acute mountain sickness.
- Source :
-
Pharmaceutical biology [Pharm Biol] 2021 Dec; Vol. 59 (1), pp. 1540-1550. - Publication Year :
- 2021
-
Abstract
- Context: Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba (Crassulaceae) is used to prevent and treat acute mountain sickness. However, the mechanisms underlying its effects on the central nervous system remain unclear.<br />Objective: To investigate the effect of Rhodiola crenulata on cellular metabolism in the central nervous system.<br />Materials and Methods: The viability and Hif-1α levels of microglia and neurons at 5% O <subscript>2</subscript> for 1, 3, 5 and 24 h were examined. We performed the binding of salidroside (Sal), rhodiosin, tyrosol and p -hydroxybenzyl alcohol to Hif-1α, Hif-1α, lactate, oxidative phosphorylation and glycolysis assays. Forty male C57BL/6J mice were divided into control and Sal (25, 50 and 100 mg/kg) groups to measure the levels of Hif-1α and lactate.<br />Results: Microglia sensed low oxygen levels earlier than neurons, accompanied by elevated expression of Hif-1α protein. Salidroside, rhodiosin, tyrosol, and p -hydroxybenzyl alcohol decreased BV-2 (IC <subscript>50</subscript> =1.93 ± 0.34 mM, 959.74 ± 10.24 μM, 7.47 ± 1.03 and 8.42 ± 1.63 mM) and PC-12 (IC <subscript>50</subscript> =6.89 ± 0.57 mM, 159.28 ± 8.89 μM, 8.65 ± 1.20 and 8.64 ± 1.42 mM) viability. They (10 μM) reduced Hif-1α degradation in BV-2 (3.7-, 2.5-, 2.9- and 2.5-fold) and PC-12 cells (2.8-, 2.8-, 2.3- and 2.0-fold) under normoxia. Salidroside increased glycolytic capacity but attenuated oxidative phosphorylation. Salidroside (50 and 100 mg/kg) treatment increased the protein expression of Hif-1α and the release of lactate in the brain tissue of mice.<br />Conclusions: These results suggest that Sal induces metabolic reprogramming by regulating the Hif-1α signalling pathway to activate compensatory responses, which may be the core mechanism underlying the effect of Rhodiola crenulata on the central nervous system.
- Subjects :
- Acute Disease
Animals
Brain drug effects
Brain metabolism
Cell Survival drug effects
Disease Models, Animal
Dose-Response Relationship, Drug
Glucosides administration & dosage
Glucosides isolation & purification
Male
Mice
Mice, Inbred C57BL
PC12 Cells
Phenols administration & dosage
Phenols isolation & purification
Rats
Signal Transduction drug effects
Altitude Sickness drug therapy
Glucosides pharmacology
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Phenols pharmacology
Rhodiola chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1744-5116
- Volume :
- 59
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Pharmaceutical biology
- Publication Type :
- Academic Journal
- Accession number :
- 34739769
- Full Text :
- https://doi.org/10.1080/13880209.2021.1992449