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Paper-based lateral flow assay using rhodamine B-loaded polymersomes for the colorimetric determination of synthetic cannabinoids in saliva.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2021 Nov 03; Vol. 188 (11), pp. 402. Date of Electronic Publication: 2021 Nov 03. - Publication Year :
- 2021
-
Abstract
- Synthetic cannabinoids are one of the many substances of abuse widely spreading in modern society. Medical practitioners and law enforcement alike highly seek portable, efficient, and reliable tools for on-site detection and diagnostics. Here, we propose a colorimetric lateral flow assay (LFA) combined with dye-loaded polymersome to detect the synthetic cannabinoid JWH-073 efficiently. Rhodamine B-loaded polymersome was conjugated to antibodies and fully characterized. Two LFA were proposed (sandwich and competitive), showing a high level of sensitivity with a limit of detection (LOD) reaching 0.53 and 0.31 ng/mL, respectively. The competitive assay was further analyzed by fluorescence, where the LOD reached 0.16 ng/mL. The application of the LFA over spiked synthetic saliva or real human saliva demonstrated an overall response of 94% for the sandwich assay and 97% for the competitive LFA. The selectivity of the system was assessed in the presence of various interferents. The analytical performance of the LFA system showed a coefficient of variation below 6%. The current LFA system appears as a plausible system for non-invasive detection of substance abuse and shows promise for synthetic cannabinoid on-site sensing.<br /> (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
- Subjects :
- Antibodies, Immobilized immunology
Cannabinoids immunology
Colorimetry instrumentation
Colorimetry methods
Humans
Illicit Drugs analysis
Illicit Drugs immunology
Immunoassay instrumentation
Immunoassay methods
Indoles analysis
Indoles immunology
Limit of Detection
Naphthalenes analysis
Naphthalenes immunology
Paper
Reproducibility of Results
Saliva chemistry
Substance Abuse Detection instrumentation
Cannabinoids analysis
Fluorescent Dyes chemistry
Polyesters chemistry
Polyethylene Glycols chemistry
Rhodamines chemistry
Substance Abuse Detection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 188
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 34731326
- Full Text :
- https://doi.org/10.1007/s00604-021-05062-y