Back to Search
Start Over
The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence.
- Source :
-
PloS one [PLoS One] 2021 Nov 03; Vol. 16 (11), pp. e0259465. Date of Electronic Publication: 2021 Nov 03 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Actinobacteria classification
Actinobacteria isolation & purification
Bacterial Proteins genetics
Bacterial Proteins metabolism
Chitinases metabolism
Genome, Bacterial
High-Throughput Nucleotide Sequencing
Hydrolysis
N-Glycosyl Hydrolases genetics
N-Glycosyl Hydrolases metabolism
Phylogeny
Plant Leaves chemistry
Plant Leaves microbiology
Glycine max metabolism
Actinobacteria physiology
Chitin chemistry
Chitinases genetics
Glycine max microbiology
Whole Genome Sequencing methods
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 16
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 34731210
- Full Text :
- https://doi.org/10.1371/journal.pone.0259465