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60Co-γ Radiation Alters Developmental Stages of Zeugodacus cucurbitae (Diptera: Tephritidae) Through Apoptosis Pathways Gene Expression.
- Source :
-
Journal of insect science (Online) [J Insect Sci] 2021 Sep 01; Vol. 21 (5). - Publication Year :
- 2021
-
Abstract
- Radiation is considered as a promising insect pest control strategy for minimizing postharvest yield losses. Among various techniques, irradiation is a method of choice as it induces lethal biochemical or molecular changes that cause a downstream cascade of abrupt physiological abnormalities at the cellular level. In this study, we evaluated the effect of 60Co-γ radiation on various developmental stages of Zeugodacus cucurbitae Coquillett and subsequent carry-over effects on the progeny. For this purpose, we treated eggs with 30- and 50-Gy radiation doses of 60Co-γ. We found that radiation significantly affected cellular antioxidants, insect morphology, and gene expression profiles. Our results indicate that in response to various doses of irradiation reactive oxygen species, catalase, peroxidase, and superoxide dismutase activities were increased along with a significant increase in the malondialdehyde (MDA) content. We observed higher mortality rates during the pupal stage of the insects that hatched from irradiated eggs (50 Gy). Furthermore, the life span of the adults was reduced in response to 50 Gy radiation. The negative effects carried over to the next generation were marked by significantly lower fecundity in the F1 generation of the irradiation groups as compared to control. The radiation induced morphological abnormalities at the pupal, as well as the adult, stages. Furthermore, variations in the gene expression following irradiation are discussed. Taken together, our results signify the utility of 60Co-γ radiation for fruit fly postharvest management.<br /> (© The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America.)
- Subjects :
- Animals
Antioxidants metabolism
Antioxidants radiation effects
Apoptosis genetics
Catalase metabolism
Catalase radiation effects
Cobalt Radioisotopes pharmacology
Insect Control methods
Insect Proteins metabolism
Insect Proteins radiation effects
Larva genetics
Larva metabolism
Larva physiology
Larva radiation effects
Longevity radiation effects
Malondialdehyde metabolism
Malondialdehyde radiation effects
Peroxidase metabolism
Peroxidase radiation effects
Pest Control methods
Pupa genetics
Pupa metabolism
Pupa physiology
Pupa radiation effects
Reactive Oxygen Species metabolism
Reactive Oxygen Species radiation effects
Tephritidae genetics
Tephritidae metabolism
Tephritidae physiology
Apoptosis radiation effects
Gamma Rays
Gene Expression radiation effects
Tephritidae radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1536-2442
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of insect science (Online)
- Publication Type :
- Academic Journal
- Accession number :
- 34718645
- Full Text :
- https://doi.org/10.1093/jisesa/ieab080