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With great power comes great responsibility: high-dimensional spectral flow cytometry to support clinical trials.
- Source :
-
Bioanalysis [Bioanalysis] 2021 Nov; Vol. 13 (21), pp. 1597-1616. Date of Electronic Publication: 2021 Oct 28. - Publication Year :
- 2021
-
Abstract
- Flow cytometry is a powerful technology used in research, drug development and clinical sample analysis for cell identification and characterization, allowing for the simultaneous interrogation of multiple targets on various cell subsets from limited samples. Recent advancements in instrumentation and fluorochrome availability have resulted in significant increases in the complexity and dimensionality of flow cytometry panels. Though this increase in panel size allows for detection of a broader range of markers and sub-populations, even in restricted biological samples, it also comes with many challenges in panel design, optimization, and downstream data analysis and interpretation. In the current paper we describe the practices we established for development of high-dimensional panels on the Aurora spectral flow cytometer to aid clinical sample analysis.
- Subjects :
- Clinical Trials as Topic
Humans
Flow Cytometry
Subjects
Details
- Language :
- English
- ISSN :
- 1757-6199
- Volume :
- 13
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Bioanalysis
- Publication Type :
- Academic Journal
- Accession number :
- 34708658
- Full Text :
- https://doi.org/10.4155/bio-2021-0201