SARS-CoV-2 surveillance in untreated wastewater: detection of viral RNA in a low-resource community in Buenos Aires, Argentina.

Authors :: Iglesias NG
Gebhard LG
Carballeda JM
Aiello I
Recalde E
Terny G
Ambrosolio S
L'Arco G
Konfino J
Brardinelli JI
Source :: Revista panamericana de salud publica = Pan American journal of public health [Rev Panam Salud Publica] 2021 Oct 18; Vol. 45, pp. e137. Date of Electronic Publication: 2021 Oct 18 (Print Publication: 2021).
Publication Year :: 2021
Abstract: Objective: To measure SARS-CoV-2 RNA in sewage in a low-resource community in order to determine if it can be considered as an estimator of changes in the prevalence of COVID-19 in the population.<br />Methods: In this descriptive observational study we collected samples of surface waters contaminated with sewage and optimized a method of purification of viral RNA using PEG concentration. We determined the amount of genetic material by quantitative real-time PCR using the CDC method for SARS-CoV-2 detection.<br />Results: We quantified viral RNA in surface waters contaminated with sewage of a low resource community and determined that temporal trends of SARS-CoV-2 in wastewater samples mirrored trends in COVID-19 active cases.<br />Conclusions: Measuring of SARS-CoV-2 RNA in sewage can be applied in low-resource communities without connection to sewers as an estimator of changes in the prevalence of COVID-19.

Language :: English
ISSN :: 1680-5348
Volume :: 45
Database :: MEDLINE
Journal :: Revista panamericana de salud publica = Pan American journal of public health
Publication Type :: Academic Journal
Accession number :: 34703463
Full Text :: https://doi.org/10.26633/RPSP.2021.137

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