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Novel Fusari- and Toti-like Viruses, with Probable Different Origins, in the Plant Pathogenic Oomycete Globisporangium ultimum .
- Source :
-
Viruses [Viruses] 2021 Sep 25; Vol. 13 (10). Date of Electronic Publication: 2021 Sep 25. - Publication Year :
- 2021
-
Abstract
- To further classify the oomycete viruses that have been discovered in recent years, we investigated virus infection in the plant-parasitic oomycete Globisporangium ultimum in Japan. Double-stranded RNA detection, high-throughput sequencing, and RT-PCR revealed that the G. ultimum isolate UOP226 contained two viruses related to fusarivirus and totivirus, named Pythium ultimum RNA virus 1 (PuRV1) and Pythium ultimum RNA virus 2 (PuRV2), respectively. Phylogenetic analysis of the deduced amino acid sequence of the RNA-dependent RNA polymerase (RdRp) showed that fusari-like PuRV1 belonged to a different phylogenetic group than Plasmopara viticola lesion-associated fusari virus (PvlaFV) 1-3 from oomycete Plasmopara viticola . Codon usage bias of the PuRV1 RdRp gene was more similar to those of fungi than Globisporangium and Phytophthora , suggesting that the PuRV1 ancestor horizontally transmitted to G. ultimum ancestor from fungi. Phylogenetic analysis of the deduced amino acid sequence of the RdRp of toti-like PuRV2 showed a monophyletic group with the other toti-like oomycete viruses from Globisporangium , Phytophthora, and Pl. viticola . However, the nucleotide sequences of toti-like oomycete viruses were not so homologous, suggesting the possibility of convergent evolution of toti-like oomycete viruses.
- Subjects :
- Base Sequence
Fungi
High-Throughput Nucleotide Sequencing
Japan
Open Reading Frames
Phylogeny
Phytophthora virology
RNA Viruses genetics
RNA, Double-Stranded
RNA-Dependent RNA Polymerase genetics
Sequence Analysis, DNA
Totiviridae genetics
Genome, Viral
Oomycetes virology
Plants microbiology
RNA, Viral classification
RNA, Viral genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 13
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 34696361
- Full Text :
- https://doi.org/10.3390/v13101931