Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents.

Authors :: Haile S
Nikiforuk AM
Pandoh PK
Twa DDW
Smailus DE
Nguyen J
Pleasance S
Wong A
Zhao Y
Eisler D
Moksa M
Cao Q
Wong M
Su E
Krzywinski M
Nelson J
Mungall AJ
Tsang F
Prentice LM
Jassem A
Manges AR
Jones SJM
Coope RJ
Prystajecky N
Marra MA
Krajden M
Hirst M
Source :: Journal of virological methods [J Virol Methods] 2022 Jan; Vol. 299, pp. 114339. Date of Electronic Publication: 2021 Oct 20.
Publication Year :: 2022
Abstract: The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.<br /> (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)

Subjects :: COVID-19 Testing
Humans
Indicators and Reagents
Magnetic Phenomena
Pandemics
RNA, Viral genetics
SARS-CoV-2
Sensitivity and Specificity
COVID-19
Nucleic Acids

Full Text Access

Tools