Back to Search
Start Over
Functional molecular switches of mammalian G protein-coupled bitter-taste receptors.
- Source :
-
Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2021 Dec; Vol. 78 (23), pp. 7605-7615. Date of Electronic Publication: 2021 Oct 23. - Publication Year :
- 2021
-
Abstract
- Bitter taste receptors (TAS2Rs) are a poorly understood subgroup of G protein-coupled receptors (GPCRs). The experimental structure of these receptors has yet to be determined, and key-residues controlling their function remain mostly unknown. We designed an integrative approach to improve comparative modeling of TAS2Rs. Using current knowledge on class A GPCRs and existing experimental data in the literature as constraints, we pinpointed conserved motifs to entirely re-align the amino-acid sequences of TAS2Rs. We constructed accurate homology models of human TAS2Rs. As a test case, we examined the accuracy of the TAS2R16 model with site-directed mutagenesis and in vitro functional assays. This combination of in silico and in vitro results clarifies sequence-function relationships and proposes functional molecular switches that encode agonist sensing and downstream signaling mechanisms within mammalian TAS2Rs sequences.<br /> (© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Details
- Language :
- English
- ISSN :
- 1420-9071
- Volume :
- 78
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- Cellular and molecular life sciences : CMLS
- Publication Type :
- Academic Journal
- Accession number :
- 34687318
- Full Text :
- https://doi.org/10.1007/s00018-021-03968-7