Back to Search
Start Over
Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation.
- Source :
-
ELife [Elife] 2021 Oct 18; Vol. 10. Date of Electronic Publication: 2021 Oct 18. - Publication Year :
- 2021
-
Abstract
- Epithelial networks are commonly generated by processes where multicellular aggregates elongate and branch. Here, we focus on understanding cellular mechanisms for elongation using an organotypic culture system as a model of mammary epithelial anlage. Isotropic cell aggregates broke symmetry and slowly elongated when transplanted into collagen 1 gels. The elongating regions of aggregates displayed enhanced cell proliferation that was necessary for elongation to occur. Strikingly, this locoregional increase in cell proliferation occurred where collagen 1 fibrils reorganized into bundles that were polarized with the elongating aggregates. Applying external stretch as a cell-independent way to reorganize the extracellular matrix, we found that collagen polarization stimulated regional cell proliferation to precipitate symmetry breaking and elongation. This required β1-integrin and ERK signaling. We propose that collagen polarization supports epithelial anlagen elongation by stimulating locoregional cell proliferation. This could provide a long-lasting structural memory of the initial axis that is generated when anlage break symmetry.<br />Competing Interests: HK, JT, RJ, JH, SS, AY No competing interests declared<br /> (© 2021, Katsuno-Kambe et al.)
- Subjects :
- Humans
Cell Proliferation
Collagen physiology
Epithelial Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 34661524
- Full Text :
- https://doi.org/10.7554/eLife.67915