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Analysis of the oligomeric state of mycobacterial membrane protein large 3 and its interaction with SQ109 with native cell membrane nanoparticles system.
- Source :
-
Biochimica et biophysica acta. Biomembranes [Biochim Biophys Acta Biomembr] 2022 Feb 01; Vol. 1864 (1), pp. 183793. Date of Electronic Publication: 2021 Oct 13. - Publication Year :
- 2022
-
Abstract
- Mycobacterial membrane protein large 3 (Mmpl3) as a trehalose monomycolate lipid transporter contributes to cell wall biosynthesis. Inhibition of Mmpl3 can suppress cell growth and lead to mycobacterial death. SQ109 is a hydrophobic inhibitor of Mmpl3. We have devised a detergent-free strategy to characterize the SQ109/Mmpl3 interaction using the Native Cell Membrane Nanoparticles (NCMN) system, a new method for extracting membrane proteins that better retains native lipids. The homogeneity of the Mmpl3 NCMN particles was confirmed with electron microscopy. The hydrophobic protein-ligand interaction analysis shown for Mmpl3 using the NCMN system may broadly apply to other membrane proteins.<br /> (Copyright © 2021. Published by Elsevier B.V.)
- Subjects :
- Adamantane chemistry
Bacterial Proteins genetics
Carrier Proteins genetics
Cell Membrane chemistry
Lipids chemistry
Lipids genetics
Membrane Proteins chemistry
Membrane Proteins genetics
Membrane Transport Proteins genetics
Mycobacterium genetics
Nanoparticles chemistry
Adamantane analogs & derivatives
Bacterial Proteins chemistry
Carrier Proteins chemistry
Ethylenediamines chemistry
Membrane Transport Proteins chemistry
Mycobacterium chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-2642
- Volume :
- 1864
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta. Biomembranes
- Publication Type :
- Academic Journal
- Accession number :
- 34655545
- Full Text :
- https://doi.org/10.1016/j.bbamem.2021.183793